Journal: Bioactive Materials

Article Title: Injectable acellular matrix microgel assembly with stem cell recruitment and chondrogenic differentiation functions promotes microfracture-based articular cartilage regeneration

doi: 10.1016/j.bioactmat.2024.10.013

Figure Lengend Snippet: Preparation and characterization of CACM and SIM@CACM microgel: a) schematic illustration on CACM extraction process from natural articular cartilage (NAC); b) GAGs, DNA and Col II retention of NAC and CACM; c) histological staining including H&E staining, Alcian blue staining and safranin O staining; d) optical images of CACM gelation; e) optical images of SIM@CACM microgels in oil phase and water phase; f) size distribution of SIM@CACM microgels in water phase. ∗∗∗P < 0.001, ∗∗P < 0.01, n = 4.

Article Snippet: Specifically, cells were fixed, blocked with 2 % BSA/PBS, and labelled in turn with rabbit polyclonal anti-Col II antibody (Servicebio, China) overnight at 4 °C, FITC-conjugated phospholipid-like protein (Servicebio, China) and DAPI (Servicebio, China).

Techniques: Extraction, Staining

Journal: Bioactive Materials

Article Title: Injectable acellular matrix microgel assembly with stem cell recruitment and chondrogenic differentiation functions promotes microfracture-based articular cartilage regeneration

doi: 10.1016/j.bioactmat.2024.10.013

Figure Lengend Snippet: In vitro chondrogenic differentiation of BMSCs cultured on SIM@CACM microgel assembly: a) fluorescence images of DAPI, F-actin and Col II; b) fluorescence intensities of DAPI, F-actin and Col II; c) immunohistochemical (IHC) staining of Col II; d) mRNA expression. ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, n = 4.

Article Snippet: Specifically, cells were fixed, blocked with 2 % BSA/PBS, and labelled in turn with rabbit polyclonal anti-Col II antibody (Servicebio, China) overnight at 4 °C, FITC-conjugated phospholipid-like protein (Servicebio, China) and DAPI (Servicebio, China).

Techniques: In Vitro, Cell Culture, Fluorescence, Immunohistochemical staining, Immunohistochemistry, Expressing

Journal: Bioactive Materials

Article Title: Injectable acellular matrix microgel assembly with stem cell recruitment and chondrogenic differentiation functions promotes microfracture-based articular cartilage regeneration

doi: 10.1016/j.bioactmat.2024.10.013

Figure Lengend Snippet: a) Safranin O-fast green staining and b) immunohistochemical staining of Col II after implanting normal saline (blank), SIM@CACM microgels, CACM microgel assembly and SIM@CACM microgel assembly respectively for 4 and 8 weeks; c) evaluation of cartilage repair performance via ICRS scoring system; d) evaluation of cartilage repair performance via O'Driscoll scoring system; NC stands for newborn cartilage, and OC stands for original cartilage.

Article Snippet: Specifically, cells were fixed, blocked with 2 % BSA/PBS, and labelled in turn with rabbit polyclonal anti-Col II antibody (Servicebio, China) overnight at 4 °C, FITC-conjugated phospholipid-like protein (Servicebio, China) and DAPI (Servicebio, China).

Techniques: Staining, Immunohistochemical staining, Saline

Journal: Bioactive Materials

Article Title: L-arginine loading porous PEEK promotes percutaneous tissue repair through macrophage orchestration

doi: 10.1016/j.bioactmat.2024.05.025

Figure Lengend Snippet: Histological analysis of the tissue around samples. (a) Representative HE staining images of tissue around the samples post implantation for 1 month (the green arrows mark the neutrophil in tissue around samples and the blue arrows mark the newly formed vessels in the tissue around samples). (b) Quantitative bone area calculated by HE (n = 3); (c) Represent Col-II staining images of bone tissue around the samples post implantation for 1 month; (d) Quantitative positive area of Col-II (n = 4). Data are presented by mean with SD. Statistical significance was calculated by one-way ANOVA analysis. **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with SPLA10 in b and d.

Article Snippet: For immunohistochemistry staining, rabbit polyclonal anti-Col-III antibody (Servicebio, GB111629, 1: 500) and rabbit polyclonal anti-Col-II antibody (Servicebio, GB11021, 1: 200), and secondary antibody, HRP-labeled goat anti-rabbit IgG (Servicebio, GB23303, 1: 200), were used to stain Col-III and Col-II, respectively.

Techniques: Staining

Journal: Polymers

Article Title: Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration

doi: 10.3390/polym14204292

Figure Lengend Snippet: Matrix deposition after 28 days of subcutaneous implantation. Representative immunohistochemical staining of the hydrogel samples for COL II. Positive protein expression stained in dark brown. The left panel in each condition shows 10× magnification pictures (scale bars represent 250 µm), whereas the right panel shows 20× (scale bars represent 100 µm).

Article Snippet: Slides were subsequently incubated overnight at 4 °C with a rabbit polyclonal antibody against COL II (Abcam, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, Expressing